©2020 by CRMN Lyon

INSTRUMENTATION

The CRMN was conceived around the installation of the first worldwide GHz spectrometer, delivered in the summer of 2009 and progressively equipped with a variety of state-of-the-art accessories (including ultra-fast magic-angle spinning probes and solution cryoprobes). After 10 years of operation this machine remains the most powerful high resolution NMR spectrometer in the world, allowing the study of a broad range of samples. In particular, it has led to major breakthroughs in the field of functionalized materials, heterogeneous catalysis, energy storage, structural biology for health, and metabolomic profiling in oncology. Around this instrument, a platform has been built up of six other 400, 500, 600, 700, and 800 (x2) MHz frequency spectrometers, offering outstanding research capabilities. Today, the center additionally hosts a unique hyperpolarized NMR platform with two 400 MHz and 800 MHz magic angle spinning (MAS) DNP systems as well as two polarizers for dissolution DNP.

 

NMR SPECTROMETERS

Bruker Prototype d-DNP Polarizer 300 MHz / 7.05 Tesla / 198 GHz equipped with:

• Brukrer AVANCE III HD

• d-DNP cabinet

• 1.2 K cryostat

• dry-pump Edwards iGX6/100M

• 2 NMR probes 1H/13C, 1H/15N


Bruker 400 MHz Ascend NMR spectrometer (DNP) equipped with:

• AVANCE III HD Bruker

• 263 GHz Gyrotron

• LT-MAS cryocabinet with automatic refill system

• sweep field unit

• 2 DNP NMR probes : 1.3 mm LTMAS HXY, 3.2 mm LTMAS HXY


Bruker 500 MHz WB Ultrashield NMR Spectromter equipped with:

• AVANCE III Bruker

• LT-MAS cryocabinet with automatic refill system

• MAS 3 unit

• BCU 2 cooling unit

• 9 solid NMR probes: 0.7mmHXY,1.3mmHX,2.5mmBBHX,2.5mmCRAMPS,3.2mmHCN,4

mm HXY, 4 mm BB HX, 7 mm BB HX, Static HX


Bruker 600 MHz Ultrashield Plus NMR spectrometer equipped with:

• AVANCE III Bruker

• SampleJet autosampler (5 racks of 96 samples and 30 spinners)

• cryo-platform

• 5 mm TXI cryoprobe HCND


Bruker 700 MHz Ultrashield NMR spectrometer equipped with:

• AVANCE III Bruker

• BCU 2 cooling unit

• HR-MAS SamplePro autosampler (50 samples)

• 4solidNMRprobes: 1.3mmHCN,2.5mmBBHX,3.2mmHCN,4mmBBHX

• 1 HRMAS probe

• 2 liquid NMR probes: TXI 5mm, BBO 5mm


Bruker 800 MHz WB/RS Ultrashield Plus NMR spectrometer (DNP) equipped with:

• AVANCE III Bruker

• 527 GHz Gyrotron

• LT-MAS cryocabinet with automatic refill system

• sweep field unit

• BCU-Xtreme cooling unit

• 2 solid NMR probes : 1.3 mm TR (HCN or HCD or HC), 3.2 mm Efree HCN

• 2 DNP NMR probes: 1.3 mm LTMAS HXY, 3.2 mm LTMAS HXY


Bruker 800 MHz Ultrashield Plus NMR spectrometer equipped with:

• AVANCE III Bruker MAS 3 unit

• SampleJet autosampler (5 racks of 96 samples and 30 spinners)

• BCU 2 cooling unit

• 5solidNMRprobes: 0.7mmHCND,1.3mmHCN,1.3mmHCND,2.5mmBBHX,3.2mmHCN

• 1 HRMAS NMR probe

• 1 liquid NMR probe: TXI 5 mm


Bruker 1000 MHz Ultrastabilized NMR spectrometer equipped with:

• AVANCE III Bruker MAS 3 unit

• BCU 1 cooling unit

• BCU 2 cooling unit

• cryo-platforme

• 6 solid NMR probes: 0.7 mm HCN, 1.3 mm HX, 1.3 mm HCN, 1.3 mm HCND, 2.5 mm HX, 3.2 mm

HCN

• 1 liquid NMR probe: BBO 5 mm

• 1 5 mm TXI cryoprobe HCND

ELECTRON PARAMAGNETIC RESONANCE

EPR EMX nano Bruker equipped with cryogenic system for low temperature measurements

DATA MANAGEMENT AND CALCULATIONS

Data and calculation server PowerEdge T630 Dell

LABORATORIES

Wetlabs for protein and nucleic acid synthesis and purification equipped with:

• Oligosynthesiser Oligopilot 10 GE Healthcare

• HPLC Agilent Infinity 1290

• ÄKTA Purifier GE HealthCare

• ÄKTA Start GE HealthCare

• Nanodrop 2000C ThermoFisher Scientific

• Autoclave Type 3850 ELV, Tuttnauer

• 2 Orbital Incubators SHKE8000-8CE, ThermoFisher Scientific

• 2 -80 freezer

• Ultracentrifuge OPTIMA XPN 100, Beckman Coulter

• Centrifugal Concentrator miVac, ThermoFisher Scientific Bioblock

• Centrifuge Sorvall RC-6+ ThermoFisher Scientific

• Cell Disruptor EmulsiFlex B15, Sodexim SAS

Wetlab for metabolomics equipped with:

• Robot TECAN Freedom EVO

• Class II Microbiological Safety Cabinet, MSC 1.2, MSC Advantage, ThermoFisher Scientific


Wetlab for chemistry equipped with:

• 2 Glove-boxes LAB Star (Eco) MBraun

• 2 Memmert ovens